Temporal variation and detection limit of an estuarine bacterioplankton community analyzed by denaturing gradient gel electrophoresis (DGGE)

Microbial Community Fingerprints by Differential Display Denaturing Gradient 1 Gel Electrophoresis ( DD - DGGE )

Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis.

Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3' ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison.

Denaturing gradient gel electrophoresis (DGGE) as a tool for identification of marine nematodes

Many phyla of marine invertebrates are difficult to identify using conventional morphological taxonomy. Larvae of a wider set of phyla are also difficult to identify as a result of conservation of morphology between species or because morphological characters are destroyed during sampling and preservation. DNA sequence analysis has the potential for identification of marine organisms to the spe...

Detecting the Effects of Fungicides on Soil Bacteria Populations using Denaturing Gradient Gel Electrophoresis (DGGE)

Rationale Fungicide applications are a necessary practice to discourage disease development in most high quality turfgrass areas. Little is known, however, about the effects that fungicides have on resident microbial community of the soils supporting turfgrass sites. Soil bacteria are responsible for important processes including pesticide degradation, nutrient cycling, and organic matter break...

Application of denaturing gradient gel electrophoresis (DGGE) for assessing fecal pollution sources at a recreational beach.

We investigated the effectiveness of Escherichia coli community fingerprinting for identifying fecal pollution sources impacting a recreational beach. E. coli in water collected from the beach, nearby creek and storm sewer outfall were enumerated using membrane filtration, while E. coli communities were characterized following polymerase chain reaction analysis and denaturing gradient gel elect...